Pristionchus pacificus Protocols

From Wikionchus

1. AFLP
2. BAC-filter hybridization
3. Buffers commonly used for worm protocols
4. Cryopreservation / Freezing stocks
5. DAPI staining
6. Dauer cultures and Purification
7. Deletion library
8. Dil Sensory Neuron staining in nematodes
9. Freezing & Thawing of nematodes
10. Immunoflourescence staining of whole animals
11. Laser ablation
12. Mapping
13. Mapping with SSLP markers
14. Medienküche
15. Microarray
16. Mutagenesis
17. Nomarski microscopy
18. Quantitative RT-PCR on Lighty cycler 480
19. RNA preparation from few worms
20. RNA purification/precipitation - using Phenol:Chloroform:Isoamyl Alcohol
21. Setting up crosses
22. Single worm lysis and PCR
23. Stock keeping
24. SSCP
25. Synchronisation of nematodes

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