Nomarski microscopy

Nomarski microscopy is the same as DIC microscopy. To mount worms for microscopy:

Place a drop of molten 4% Agar Noble onto a microscopy slide.
Place a second slide over the first one in order to compress the Agar into a flat pad.
Let Agar cool out.
Remove top slide by sliding or lifting it off.
The amount of buffer used is Voodoo. Some say 2.7 µl, other dont care, others make "oceans" on their slides. As a starting point place 4 µl of PBS or M9 on top of the Agar pad. You might use sodium azide to anesthesize the worms.
Pick animals into the drop of buffer.
Place coverslip on the Agar pad with buffer and worms.
Place into the slide holder of a microscope.
Do you remember how to do Köhler illumination?
Don´t forget that we have objectives that need oil and others that must be used without oil.
Clean objectives and condensor with a green Kimwipe after use.

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