NGM

From Wikionchus

NGM stands for Nematode Growth Medium. It is in principle a LB plate containing some salts and importantly cholesterol. Nematodes can not synthesize cholesterol de novo, which is why it has to be supplied in the plates.

To make 1 l NGM agar:

• NaCl 3g

• Bacto-Peptone 2.5g For rich NGM: 7.5g (supports long-term bacterial

growth, less burrowing)

• agar 17g - 21 g

• autoclave in 1-liter dH2O

Cool to 55 °C and add (using sterile technique and swirling):

• 1 mL cholesterol (5 mg/mL in ethanol)

• 1 mL 1 M CaCl2

• 1 mL 1 M MgSO4

• 25 mL 1 M KH2PO4, pH 6.0

• Pour plates about half-full, and flame the agar surface to remove air bubbles (or worms will

burrow). Let dry at least one day prior to seeding with OP50 E. coli.