Getting started

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For breeding Pristionchus you'll need:

  • 6cm petri-dishes with NGM-agar
  • OP50 cultures for feeding: spot the NGM-plates with 250-500 ul of an OP50 overnight culture (alternatively you can also try potatoes, rotten meat etc...).
  • A dissecting microscope
  • a worm pick: flatten the tip of some platin wire with a hammer and melt it into a glass pasteur pipette.
  • an ethanol burner to sterilize your pick
  • professional worm breeders have a room that has constant 20° C and incubators at 15°C and 25°C

See also:

stock keeping



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